No abstract

This study aimed to determine the accuracy of assessing stallion sperm motility using a light microscope, a cell phone camera, and a free computer-assisted semen analysis (FCASA) package for ImageJ. The total motility of frozen (n=22) and cooled (n=48) equine semen was determined by FCASA and compared to the results of subjective visual analysis (SVA) by two technicians. Frozen samples were also evaluated by a commercial computer-assisted semen analysis (CCASA) system. The Friedman test revealed no significant differences (P>0.05) between cooled samples analyzed by FCASA (38.0) and SVA (technician 1, 40.0; technician 2, 40.0), nor between frozen samples analyzed by FCASA (23.36 ± 15.9), SVA (25.5 ± 18.8 and 25.8 ± 18.5), and CCASA (25.2 ± 18.3). However, mean FCASA results were underestimated by 7.2% compared with CCASA. The correlation between FCASA and CCASA was significant and strong (P<0.0001, r=0.95). Chi-squared tests indicated that FCASA provided similar results (P=0.14) to the reference method (CCASA), but SVA had lower accuracy (P=0.04). ImageJ analysis of cell phone videos captured under a light microscope can be used for estimation of stallion sperm motility with comparable accuracy to commercial systems.

No abstract

The effect of the contrast medium Visotrast 370 on the water content and volume of erythrocytes, and on the electrolyte levels of plasma and erythrocytes during I-V urography was examined. The measurements were carried out at one, ten and 30 minutes after injection. One minute after injection there was a significant reduction in the water content and volume of the erythrocytes. The levels of sodium and potassium ions in the plasma were reduced ten minutes after contrast injection, but increased in the erythrocytes. After 30 minutes, significant changes could no longer be demonstrated. Possible causes for these changes and their effects on other organs are discussed. Certain practical conclusions are drawn.