Airborne virus particles of vaccinia, influenza, Venezuelan equine encephalomyelitis, and poliomyelitis have been tested for viable survival in the dark at controlled temperatures and relative humidity (R.H.) for up to 23 hr. after spraying.Viable survival at each R.H. level was better at lower temperature than at higher temperature. Poliomyelitis virus showed best survival at high R.H.; the other three viruses survived best at low R.H.

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The distribution of inhaled bacterial aerosols has been studied in guinea-pigs, monkeys and mice, using Bacillus subtilis spores labelled with radiophosphorus. Particle sizes from about 1 to 12μ have been used.The guinea-pig shows the expected change of distribution with particle size; the proportion retained in the head increases with increased particle size. The figures correspond closely with those for man. Monkeys show similar results but are more irregular.The subsequent fate of the retained particles has been studied and accords with what is known about ciliary removal.The work is intended to link with parallel investigations of respiratory infection in closely similar conditions, and its implications are discussed in a paper on that subject (Druett et al. 1953).

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