The fingernail clam, Pisidium coreanum, has been traditionally consumed raw as a so-called drug therapy by patients with bone fractures in Korea. The present study was designed to determine the possible occurrence and, if present, the prevalence of Echinostoma cinetorchis in P. coreanum collected at a local site, and to determine the susceptibility of the clams in the laboratory to infection with miracidia and cercariae of E. cinetorchis. No cercariae or metacercariae of E. cinetorchis were observed in field-collected P. coreanum clams. In susceptibility experiments with laboratory-reared clams, individuals exposed to miracidia of E. cinetorchis did not release cercariae by 20 days after exposure; necropsy of exposed clams failed to show development of any sporocysts or rediae. To confirm the possibility of these clams serving as an experimental second intermediate host of E. cinetorchis, 20 of them were exposed to E. cinetorchis cercariae from experimentally infected Segmentina hemisphaerula that had been previously exposed to miracidia of E. cinetorchis; all exposed clams became infected. Metacercariae from clams at 14 days postinfection were fed to rats, and adult worms were recovered from the ileocecal regions. This is the first report of P. coreanum serving as second intermediate host of E. cinetorchis.

SummaryWe have investigated freshwater cyprinid fish for its current infection status with plerocercoid of ligulid tapeworm in the Republic of Korea. A total of 6,049 (517 Euiam Lake and 4,071 Hoengseong Lake in Gangweon-do and 1,461 Chungju Lake in Chungcheongbuk-do) fish were examined by dissecting the peritonial cavity between March 2015 and December 2016. Totally 45 (0.74 %) fish in of 5 (26.3 %) species (8 Squalidus japonicus coreanus, 6 Squalidus gracilis majimae, 7 Opsariichthys uncirostris amurensis, 15 Zacco platypus and 9 Erythroculter erythropterus) were infected with plerocercoids of ligulid tapeworm. The infection density with plerocercoids in Erythroculter erythropterus was 12 – 26 per fish infected in Erythroculter erythropterus, and 1 – 2 in other 4 fish species. The plerocercoid was ivory-white and 26.2 – 57.8 cm long. The prevalence value in this survey was 0.9 % (45/6,049). The genetic analysis in this study was conducted to identify plerocercoid species. Based on genetic analysis with data in GenBank, these plerocercoids were identified as the L. intestinalis.