An efficient protocol for organogenesis through leaves has been established for Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze, a highly valuable medicinal plant. The leaf explants produced microshoots on MS basal medium when fortified with cytokinins and auxins. A combination of 6-benzylaminopurine (BAP) at 0.5mg/l and naphthaleneacetic acid (NAA) at 0.2mg/l resulted in the induction of high frequency microshoots in 30 days. The microshoots were successfully subcultured for shoot elongation and eventually for rooting on MS medium supplemented with indole-3-butyric acid (IBA) at 0.5mg/l. The regenerated plantlets were hardened under greenhouse conditions and transferred to garden, resulting in a 90% survival rate.

Objective: To evaluate the potential aspects of biologically synthesized silver nanoparticles (AgNPs) mediated by Broussonetia papyrifera against the human pathogens. The same is acknowledged to have high efficiency in the field of Pharmaceutical industry. Methods:The 1 mM of AgNO 3 is prepared and mixed with an appropriate volume of plant extract and reaction volume was made up to 100 ml. The physical characterization of AgNPs was done. The antimicrobial activity was done against dread pathogens. Cytotoxic activity of the AgNPs was investigated against breast and lung cancer cell lines. Results:The field emission scanning electron microscopy and energy dispersive X-ray spectroscopy of the microscopic level showed the particle surface measurements around 44-50 nm. The X-ray powder diffraction investigations are being an evidence for the crystalline structure of the AgNPs with 30 nm. The bacterial pathogen Rhodococcus rhodochrous showed the maximum zone of inhibition (11.8±0.447). The A549 human lung cancer cell line and MCF-7 human breast cancer cell line were tested against the toxicity of AgNPs. The toxicity of AgNPs was valued and corresponding inhibitory concentration for lung cancer (A549) is 12.95±0.05 µg/mL and breast cancer (MCF-7) is 10.75±0.05 µg/mL, respectively. Conclusion:This research denotes that biomolecules derived AgNPs have a larger impact as antimicrobials in the biomedical field. Since the aggressive chemicals are not involved AgNPs production, these bio-substances can of alternative medicine to resistant once. The in-vitro experiments exhibit the therapeutic effect of this AgNPs based on the ambient concentration on the process.

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