To evaluate the effect of different doses of Nano-urea on yield attributes and yield of pearl millet a field experiment was conducted at Eastern Block Farm of Tamil Nadu Agricultural University, Coimbatore during the Summer of 2022. This field experiment was laid out in Randomized Block Design (RBD) with ten treatments and three replications. In this experiment different doses of Nano-urea were applied along with the Recommended Dose of Nitrogen (RDN) as per the Crop Production Guide, 2020 of TNAU, Coimbatore. The study revealed that yield of pearl millet was significantly differed with treatments imposed. Among these treatments, application of 50% RDN as basal + 0.5% Nano-urea foliar spray @ panicle initiation and booting stage recorded a significantly higher number of productive tillers plant-1 (3.79), earhead length (26.82 cm), earhead girth (7.9 cm), number of grains earhead-1 (2,462), test weight (15.64 g), grain yield (3,314 kg ha-1) and stover yield (5,136 kg ha-1). Which was at par with treatment 50% RDN as basal + 25% RDN @ panicle initiation + 25% RDN @ booting stage (T10).