Understanding the anti-agglomerant (AA) contributions to the physicochemical properties of waxy petroleum emulsions can provide improvements on handling problems caused by hydrates during petroleum production. This work evaluated the stability of a reference water-in-oil (W/O) emulsion perturbed by the presence of commercial hydrate AAs and ammonium quaternary compounds. The results showed that emulsion mean droplet size was not sensitive to the presence of these chemicals. Both W/O emulsions, with and without AA addition, were stable under a gravitational field. The amount of water separated during the stability experiments (bottle test under a centrifugal field) increased for emulsions containing a higher content of AA/quaternary ammonium compound. In a specific concentration of AA, stable W/O emulsion can no longer be obtained and an unstable liquid dispersion was formed, resembling emulsions near the transitional inversion point. In a dynamic experiment, monitored by in situ near-infrared spectroscopy (NIR), a transitional emulsion inversion was performed by adding quaternary ammonium compound to the emulsion.