No abstract

6 minutes after Joduron | 70% (0.5 ml/kg), Biligrafin | 30% (0.4 ml/kg), Urografin | 60% (0.5 ml/kg), Urovison | 58% (0.5 ml/kg), Urovist | 65% (0.75 ml/kg) and Conray | 70% (0.5 ml/kg) (within 1 minute injected) the level of circulating histamine in plasma is increased from less than 1.0 ng/ml to 2.0-2.5 ng/ml. The elevated levels return to normal within 20 minutes, in the case of Joduron | 70% and Biligrafin | 30% slower than after the other contrast media. Urivison | 30%, infused over 4 minutes (total dose 4 ml/kg), as dextrose 50%-solution (0.5 ml/kg) failed to change histamine concentration in plasma. Obviously the histamine liberating effect of the contrast media is a specific effect of these agents.

A kininogenase was purified from human plasma, after activation by contact with quartz. Fresh human plasma was stirred with quartz powder and the adsorbed protein was eluted from the quartz with IM NaCl. The kininogenase con-'tained in the eluate was purified by ammonium sulphate fractionation and preparative isoelectric focusing. Up to this stage the enzyme was bound to non-inhibitory -globulin. The aggregate had an apparent molecular weight of 180000 (gel filtration) and IP 4.7. The enzyme proper was released and separated from the -globulin during passage through a column of DEAE-cellulose in buffer of low ionic strength.The separated kininogenase migrated as a fast -globulin during electrophoresis on cellulose acetate (pH 8.6). The molecular weight was 97000 (gel filtration) and the IP 7.9-9.

No abstract

No abstract