Comparison of resolving power and separation time in thermal field-flow fractionation, hydrodynamic chromatography, and size-exclusion chromatography. Stegeman, G.; van Asten, A.C.; Kraak, J.C.; Poppe, H.; Tijssen, R.
Published in: Analytical Chemistry
DOI:10.1021/ac00079a033Link to publication
Citation for published version (APA):Stegeman, G., van Asten, A. C., Kraak, J. C., Poppe, H., & Tijssen, R. (1994). Comparison of resolving power and separation time in thermal field-flow fractionation, hydrodynamic chromatography, and size-exclusion chromatography. Analytical Chemistry, 66, 1147. DOI: 10.1021/ac00079a033
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Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. The most important chromatographic separation methods for characterizing the molecular mass distribution of polymers are thermal field-flow fractionation (TFFF), hydrodynamic chromatography (HDC), and size-exclusion chromatography (SEC). A comparison of the capabilities of these methods is of great value to select the best technique for a particular analytical purpose.For an objective comparison, the choice of the performance parameters is crucial. This choice is obviously rather arbitrary because the performance of a separation method can be judged from many different viewpoints. As the goal of each method is to obtain a highly accurate molecular mass distribution in the shortest possible time, we regard the resolving power and the analysis speed as the most important performance features just as in nearly all other chromatographic techniques. Investigating these two aspects will therefore be the main focal point in this study. Other important performance criteria that can be put forward are the peak capacity, the dynamic molecular mass range, and the useful separation range. In this respect also convenience and technical feasibility may be mentioned.Yau and Kirkland compared the capabilities of sedimentation FFF, HDC, and SEC.' They evaluated several features including resolving power, dynamic range of macromolecular size, absolute size range, and peak capacity. To obtain a common basis for comparison, they derived performance parameters which were normalized in terms of fractional differences in solute size. These parameters could be applied to methods for size separation in general. Literature data on se...
