In cancer patients, infection is the most significant and continuous problem. This study was done to show the spectrum of bacteria and sites of isolation in febrile neutropenic cancer patients. A 1-year study of all isolates was conducted from various sites in our hospital. All the samples were processed, and isolates were identified as per CLSI guidelines. The commonest organism isolated was Escherichia coli among Gram negative organisms and Staphylococcus aureus among Gram positive organism. Out of 76 isolates 49 were Gram negative and 27 were Gram positive. The pattern of infectious agents has changed in neutropenic patients over time and this postulates the need of other studies to give the most up to date insight of the causative organism to the physician.