The aim of this study is to evidence the efficacy of Methanolic extract of sphaeranthus amaranthoides (MESA) against hormonal changes induced by nicotine in male adult wistar albino rats. 24 Adult male Wistar albino rats, weighing about 170 ± 25 gm were divided into four groups and treated with Normal saline ( Control), Nicotine (NT), Nicotine followed by 250mg MESA Dose 1 + NT and Nicotine followed by 500mg MESA Dose 2 + NT. All the animals were treated for 48 days continuous treatment. Serum Hormone levels were measured at 20th day, 40th day and 48th day. The hormonal changes were verified by a noticeable reduction in serum levels of testosterone, follicle-stimulating hormone (FSH), and increase in luteinizing hormone (LH), and prolactin. Our findings propose that MESA has a beneficial effect in reversing the hormonal damages induced by Nicotine by acting on the cell of various stages of spermatogenesis.