Sample collection in livestock animals is difficult because choosing proper device for collecting samples is uncertain. Flocked swabs are the most recent advancement in single-use specimen collecting equipment. Flocking is the application of multi-length fibres to an adhesive-coated surface to improve sample collecting method and process. Since the onset of the animal collection swabs, nylon flocked technology has become a new tool in the field of healthcare especially diagnostic. Advantages of non-invasive methods include painless collection ensuring the best possible animal welfare. Nylon flocked swabs can be utilized for a host cell sample collection providing high quality and enough amount of DNA. In the present study, through nasal swab high amount of DNA was identified for goat (124.03 ng/µl) as compared to bovine (96.734 ng/µl) and pig (87.638 ng/µl). Thus nylon flocked nasal swabs showed a good performance for Veterinary diagnosis, although this would be an alternative promising specimen collecting tool.