Chimeric oligo(ribo-deoxyribo)nucleotides with an internucleotide pyrophosphate bond are novel probes for regiospecific hydrolysis of RNA by RNase H. It has been shown that the use of d(TGTGTAT)ppGC-CAU leads to unique hydrolysis of the TMV RNA fragment pAAUGGCAUACAC between C,, and A,, .
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