Soil pollution by heavy metals as a result of mining activities is increasingly taking place. Once accumulated in soil, the heavy metals can then be dispersed, with serious effects on the environment and human health. It is therefore necessary to minimize, or even remove, all heavy metals from polluted areas, and one of the environmentally friendly and sustainable methods to do so is phytoremediation. A greenhouse pots experiment was conducted to evaluate the phytoremediation capacity of Silphium perfoliatum L. plants, in the vegetative growth stages, on a soil polluted with Cu, Zn, Cr and Pb, taken from a former mining area compared to an unpolluted soil (Us). The initial heavy metal content of polluted soil (Ps) was 208.3 mg kg−1 Cu; 312.5 mg kg−1 Zn; 186.5 mg kg−1 Cr and 195.2 mg kg−1 Pb. This shows that for Cu and Pb, soil concentrations exceed the intervention threshold, and for Zn and Cr, they are above the alert threshold. The removal efficiency, bioaccumulation factor, translocation factor, metal uptake and contamination factor index of Cu, Zn, Cr and Pb by S. perfoliatum L. were quantified to determine the bioremediation success. The data show that plants grown in Ps accumulated a significantly higher amount of Cu by 189% and Zn by 37.95% compared to Us. The Cr and Pb content of the plants recorded a progressive and significant increase from one developmental stage to another, being more intense between three and five leaves.
The purpose of the experiment was to highlight the germination of sunflower seeds affected by the presence of saline stress and the identification of tolerant genotypes. The biological material was represented by sunflower cvs. (Helianthus annuus L.): Coril, Select, Santiago and Fundulea-206. To simulate the saline conditions, germination solutions of sodium chloride (NaCl) were used with concentrations corresponding to the osmotic pressures -6 and -10 atm and the control seed hydration was performed with distilled water. Determination of seed germination, growth of seedling, percentage of plumules dry matter, chlorophyll content and free proline were performed. The experimental data obtained suppose the existence in the assimilation apparatus of sunflowers seedling subjected to stress a competitive chlorophyll/free proline biosynthesis processes. The experimental results regarding the effect of salinity on seed germination and seedling growth revealed important differences between genotypes. The radicle growth in the germination process were strongly affected by saline excess, with significant differences between cultivars. Saline stress results in significant reductions in the amount of chlorophyll, and high levels of free proline. It can be observed that with the increase of the stress level the percentage of the dry matter increases, indicating an accentuated water deficit.
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