Eight yeast strains that asexually reproduce by cell fission were isolated from bee bread of different solitary bees in Germany. DNA sequence analysis revealed that the strains shared the same sequence in the D1/D2 domain of the nuclear large subunit (LSU) rRNA gene with a strain that was previously isolated from a fig snack from Spain. The closest related type strain was that of Schizosaccharomyces octosporus, which showed 98.2% sequence similarity (11 substitutions) with the new strains. By clone sequence analysis of the internal transcribed spacer (ITS) region (ITS1, 5.8S rDNA, and ITS2) a total of nine different copy types were identified. The new strains differed from S. octosporus by approximately 31% in the ITS region. Sequence analysis of the RNAse P gene further supported the description of a new species. The strains isolated during this study show some phenotypic characteristics that separate them from the closest related species, S. octosporus and S. cryophilus. Since all strains showed true osmophily the name of the new species is S. osmophilus (holotype: CBS 15793T; isotype: CLIB 3267 T = NCAIM Y.02225 T, MycoBank no.: MB829586).

No abstract

No abstract

No abstract

Following a two-step enrichment in methanol containing broth, methylotrophic yeast strains were isolated from about 45% of the leaf samples collected from broad leafed deciduous trees and from herbs in Hungary. During the enrichment process protists predating the yeasts were observed. Based on standard phenotypical tests and the D1/D2 domain sequences of the large subunit (26S) rDNA of the yeast strains recovered from the phylloplane, some of them represent previously unknown species. The description of a new methylotrophic yeast species, Ogataea allantospora [type strain: NCAIM Y.01822(T) (CBS 10576, NRRL Y-48267)], isolated from phylloplane is given. The proposed new species is the first member of the genus which forms allantoid ascospores, therefore the emendation of the diagnosis of the genus Ogataea Yamada, Maeda & Mikata is proposed.