The fourth generation of the Sloan

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The growth of Chlamydomonas reinhardtii microalgae cultures was successfully monitored, from classic off-line optical techniques (optical density and fluorescence) to on-line analysis of digital images. In this study, it is shown that the chlorophyll fluorescence ratio F685/F740 has a linear correlation with the logarithmic concentration of microalgae. Moreover, with digital images, the biomass concentration was correlated with: the luminosity of the images through an exponential equation, and the length of penetration of a superluminescent blue beam (λ=440 nm), through an inversely proportional function. Outcomes of this study are useful to monitor both research and industrial microalgae cultures.

The growth of Chlamydomonas reinhardtii microalgae cultures was successfully monitored, using classic off-line optical techniques (optical density and fluorescence) and on-line analysis of digital images. In this study, we found that the chlorophyll fluorescence ratio F685/F740 has a linear correlation with the logarithmic concentration of microalgae. By using digital images, the biomass concentration correlated with the luminosity of the images through an exponential equation and the length of penetration of a super luminescent blue beam (λ = 440 nm) through an inversely proportional function. The outcomes of this study are useful to monitor both research and industrial microalgae cultures.