Technological tools, teaching materials and students’ acceptance are key aspects for online education at current times. The use of computer algebra systems (CAS) may improve students’ perception about online learning as it offers an interactive environment where academic achievement could increase. Math software Wolfram Mathematica under its cloud version was studied for learning second semester multivariate calculus online. A quasi-experiment was conducted with control and experimental groups. Pre-tests were carried out to evaluate the similarity of groups prior treatment and post-tests to assess scores after students learnt derivatives in multivariable calculus. Two-sample t-test was used to determine how similar the means of the control and experimental groups were for pre and post-tests’ scores. Experimental group scored significantly better than control group demonstrating the positive effect this software had on multivariate calculus learning. Students from experimental group answered a survey to evaluate in more detail the experience. Results indicate that math software should be implemented in calculus courses during online education.