A rapid, sensitive and specific method for quantifying piracetam in human plasma using Piracetam d-8 as the internal standard (IS) is described. The analyte and the IS were extracted from plasma by one-step precipitation of protein using an acetonitrile (100%). The extracts were analyzed by high-performance liquid chromatography coupled with electrospray tandem mass spectrometry (HPLC-MS/MS). The method had a chromatographic run time of 3.8 min and a linear calibration curve over the range 0.5-50 µg/mL (r > 0.99). This LC-MS-MS procedure was used to assess the bioavailability of two piracetam formulations: piracetam + l-carnitine (Piracar®; 270/330 mg tablet) and piracetam (Nootropil®; 800 mg tablet) in healthy volunteers of both sexes. The geometric means with corresponding 90% confidence interval (CI) for test/reference percentage ratios were 88.49% (90% CI = 81.19 - 96.46) for peak concentration/dose and 102.55% (90% CI = 100.62 - 104.51) for AUCinf /dose. The limit of quantitation of 0.5 µg/mL is well suited for pharmacokinetic studies in healthy volunteers. It was concluded that piracetam (Piracar®; 270/330 mg tablet) has a bioavailability equivalent to the piracetam (Nootropil®; 800 mg tablet) formulation with regard to both the rate and the extent of absorption.

Promover saúde bucal consiste em capacitar uma população para melhorar a sua técnica de higiene oral, qualidade de vida e de saúde de maneira geral. Os estudantes de odontologia serão os futuros promotores de condições favoráveis para que se torne possível manter a saúde bucal. O objetivo desse estudo foi avaliar a efetividade da instrução de higiene bucal em alunos do primeiro ano do curso de graduação em Odontologia da Universidade Metropolitana de Santos-SP. Foram avaliados 34 participantes por meio de sessões de evidenciação de placa bacteriana através do Índice de O`Leary e Índice Periodontal de Sangramento a Sondagem. Os Índices foram realizados entre e após a orientação e motivação de higiene bucal. Verificou-se que após adquirido os conhecimentos, os Índices de placa bacteriana e sangramento reduziram e a maioria dos alunos responderam satisfatoriamente na adoção de novos hábitos de higiene bucal saudáveis.

Background: Dental biofilm accumulation and poor personal oral hygiene are known major risk factors for gingivitis and halitosis. However, it is not clear how studies compare the effectiveness of hygiene regimens, associated with outcomes centered on patients. Methods: A randomized, blind, controlled clinical trial involving 58 participants aged from 12 to 17 years, who search the Department of Pediatric Dentistry of Universidade Metropolitana de Santos , will be conducted. Immediately, the volunteers will be inserted into Group 1 (commercially available hygiene regimen) or Group 2 (tooth brushing alone). In Group 1, participants will receive Colgate Total 12 toothpastes, Plax mouthwashes and Colgate Ultrasoft toothbrushes, while Group 2 will use Colgate Cavity Protection toothpastes and Colgate Ultrasoft toothbrushes. The interventions will be conducted in the periods of 1, 3, and 6 months after the baseline, when the evaluations will also be performed. Biofilm and halitosis indexes will be evaluated. Data regarding discomfort, satisfaction and the socioeconomic/individual characteristics will also be computed. Discussion: Although toothbrushing has shown positive effects in decreasing biofilm and in gingival health, there is no comparison in the literature of different brushing regimens with halitosis measurement in adolescents. In addition, the effectiveness of these protocols would be confirmed from the acceptability of the volunteers.

Objective: The objective of this research was to evaluate stress in teachers in the five areas of Education (Early Childhood Education, Elementary School I and II, High School and University). Methods: A total of 125 teachers from Baixada Santista, SP, Brazil, were included. Physical and psychological stress in the their lives were evaluated, using the Marilda Lipp Stress Symptom Inventory (lSSI) test. Symptoms were assessed in the first 24 hours, 1 week and after 1 month. It was applied remotely, in a single application, from April to June 2021. Results: Out of the total of 125 teachers, 92 had stress and 33 had no stress. Out of the 111 female participants, 84 had stress and 27 had no stress, whereas out of the 14 male participants, 8 had stress and 6 had no stress. Of the 92 participants that showed stress, 5 participants were in the Alert Phase, 49 participants were in the Resistance Phase and 38 participants were in the Exhaustion Phase. 39 participants had Physical symptoms, 5 participants had Psychological symptoms and 48 participants had both symptoms. Conclusion: It is important to reflect on the importance of stress prevention within educational institutions, aiming to provide a better quality of life for teachers.