Ocular infection with Toxoplasma gondii causes toxoplasmosis in mice. However, following ocular infection with tachyzoites, the cause of the accompanying progressive changes in hippocampal-dependent tasks, and their relationship with the morphology and number of microglia, is less well understood. Here, in 6-month-old, female BALB/c mice, 5 μl of a suspension containing 48.5 × 106 tachyzoites/ml was introduced into the conjunctival sac; control received an equal volume of saline. Before and after instillation, all mice were subject to an olfactory discrimination (OD) test, using predator (cat) feces, and to an open-field (OF) task. After the behavioral tests, the animals were culled at either 22 or 44 days post-instillation (dpi), and the brains and retinas were dissected and processed for immunohistochemistry. The total number of Iba-1-immunolabeled microglia in the molecular layer of the dentate gyrus was estimated, and three-dimensional reconstructions of the cells were evaluated. Immobility was increased in the infected group at 12, 22, and 43 dpi, but the greatest immobility was observed at 22 dpi and was associated with reduced line crossing in the OF and distance traveled. In the OD test, infected animals spent more time in the compartment with feline fecal material at 14 and at 43 dpi. No OD changes were observed in the control group. The number of microglia was increased at 22 dpi but returned to control levels by 44 dpi. These changes were associated with the differentiation of T. gondii tachyzoites into bradyzoite-enclosed cysts within the brain and retina. Thus, infection of mice with T. gondii alters exploratory behavior, gives rise to a loss in predator’s odor avoidance from 2 weeks after infection, increased microglia number, and altered their morphology in the molecular layer of the dentate gyrus.

bilateral t-test, p = 0.445752). We concluded that cell proliferation in response to stimulation by the enriched environment is differentially expressed in the telencephalon and tectal areas of Pterophyllum scalare.

Objetivo: descrever o perfil clínico-epidemiológico do atendimento no Centro de Referência em Imunobiológicos Especiais de Hospital na Amazônia.Método: descritivo de abordagem quantitativa e analisado por meio da estatística descritiva e inferencial. O Sistema de Informação do Centro de Referência para Imunobiológicos Especiais (antigo) e Sistema de Informação do Programa Nacional de Imunização (atual) foram as bases utilizadas, entre 2006 e 2016.Resultados: foram administradas 77.077 doses de imunobiológicos; 25,2% corresponderam à vacina influenza inativada; insuficiência renal crônica foi a principal indicação (19%). O sistema atual registrou 18.267 doses de imunobiológicos administrados entre os anos de 2014 a 2016; sendo 37,8% correspondente à vacina influenza inativada; não foram informadas 42,5% das indicações, sendo HIV/AIDS 18,2%. Infectologia foi a especialidade que mais encaminhou para vacinação (18,3%).Conclusão: a acurácia dos dados foi comprometida pela falta de completude nos sistemas de informação. Observou-se subutilização do serviço pela população local.