In order to examine possible gender differences in work calling and work spirituality, 68 university academic faculty members responded to self-report multidimensional measures of these constructs. No gender differences were found for the attribution of the source of a transcendent summons, with a majority of respondents indicating internal potential, society’s needs, destiny, and family tradition as sources of this summons. MANOVA indicated that women had higher scores in comparison with men for two dimensions of calling - search for a transcendent summons and prosocial motivation – and for two dimensions of work spirituality - engaging work and mystical experience at work dimensions of work spirituality.