For efficient clonal reproduction of Vaccinium corymbosum L. of Blue Berry variety, the surface explant sterilization approaches have been developed - the apical part of the stem and stem segments using “Belizna” commercial bleach solution and nutrient media for explant cultivation have been selected. The maximum survival of the explants (100%) was observed during sterilization with a 15% of “Belizna” bleach solution with an exposure time of 15 minutes. The nutrient media Murasige-Skuga, WPM and Anderson were compared for effective breeding. The highest breeding rate of blueberry was on WPM medium with the addition of zeatin and indolyl-3-butyric acid. The maximum number of axillary shoots per explant was 3.80, with an average length of 3.26 cm, obtained on WPM medium containing 1.0 mg/l of zeatin and 0.1 mg/l of indolyl-3-butyric acid. It has been shown that with an increase in the number of passages, growth rates improve. The maximum increase in the number of healthy axillary shoots is observed at the fourth passage, while the phenomenon of vitrification begins to appear from the fifth passage.

This paper discusses a method of micropropagation of Vaccinium corymbosum L. cv. Blue-Berry. The results showed that WPM supplemented with 1,0 mg/l of zeatin in combination with 0,1 mg/l of indolyl-3-butyric acid was more effective for the multiplication of blueberry axillary shoots. The maximum increase in the number of healthy axillary shoots was observed in the fourth subculture, whereas the phenomenon of hyperhydration (vitrification) began to appear in the fifth subculture. In addition, it was established that the presence of indolyl-3-butyric acid and 1,0 g/l of activated charcoal in the nutrient medium lead to the development of good root system of the Vaccinium corymbosum cv. Blue-Berry. The highest mean number of roots formed per explant was obtained on WPM medium, supplemented with 0,5 mg/l indolyl-3-butyric acid after 10 weeks. Acclimatization of in vitro regenerated plantlets of Vaccinium corymbosum with a developed root system in ex-vitro conditions (pH 3,5-4) showed a 100% survival rate.