Poly (brilliant blue) modified carbon paste electrode was fabricated for the detection of dopamine in the presence of large excess of ascorbic acid and uric acid in phosphate buffer solution of pH 7.4. The redox peaks obtained at poly (brilliant blue) modified carbon paste electrode shows good electrocatalytic activity towards the oxidation of dopamine. From the study of scan rate variation the electrode process was found to be adsorption controlled. The limit of detection of dopamine was found to be 6.7 × 10 -7 M. and the simultaneous study shows the good result with peak to peak separation between dopamine and other two analytes ascorbic acid and uric acid by both cyclic voltammetry and differential pulse voltammetric techniques.