Dipsacus asperoides is a traditional and endemic medicinal herb in China. Its roots was considered top grade herb as early as in the Shen Nong Herbal Classic. In this study, we assembled and analyzed the complete chloroplast genome of D. asperoides. The complete chloroplast genome is 160,481 bp in length, exhibiting a large single-copy region (88,546 bp), a small single-copy region (19,671 bp), and two inverted-repeat regions (26,132 bp in each one). The chloroplast genome of D. asperoides contains 133 genes, including 89 protein-coding genes (PCGs), 36 transfer RNA (tRNAs), and eight ribosome RNA (rRNAs). The overall nucleotide content of the chloroplast genome is A of 30.2%, T of 31.0%, C of 19.7%, and G of 19.1%, with a total AT content of 61.2% and GC content of 38.8%. However, the phylogenetic Maximum-Likelihood (ML) analysis based on the amino acid sequences of 89 PCGs from 14 species chloroplast genome that D. asperoides is closely related to Dipsacus asper. This study can be used for medicinal herb value research and clinical drug development.

Platycladus orientalis belongs to the family Cupressaceae that the branches and leaves is an important Traditional Chinese Medicine in China. In this article, the complete chloroplast genome of P. orientalis was studied and illustrated to add the more genetic information. The chloroplast genome of Platycladus orientalis is 1127,113 bp in length as the circular, which exhibits 120 genes, including 83 protein-coding genes (PCG), 33 transfer RNA genes (tRNAs) and 4 ribosomal RNA genes (rRNAs). The overall nucleotide composition of chloroplast genome is: 32.1% of A, 33.2% of T, 17.9% of C, 16.8% of G and the total AT content of 65.3% and GC of 34.7%. Phylogenetic relationship shown that Platycladus orientalis is more closely related to Thuja standishii on genetic relationship using the Maximum-Likelihood (ML) method. The chloroplast genome may contribute to the medicinal valuable and evolutionary studies of this species. ARTICLE HISTORY