The eukaryotic class II polypeptide chain release factor (eRF3) is an eRF1- and ribosome-dependent GTPase involved in translation termination of protein biosynthesis. eRF3 is a multifunctional protein that is also involved in chromosomal segregation and cytokinesis during mitosis. Survivin is a member of the inhibitor of apoptosis protein (IAP) family that is involved in the organisation of spindle and cell apoptosis. Interaction between survivin and eRF3a-F3 or eRF3b, encoded by the GSPT1 and GSPT2 genes, respectively, was confirmed using yeast two-hybrid (Y2H) and pull-down assays in vitro, and co-immunoprecipitation in vivo. The domains involved in the formation of the survivin-eRF3s complex have been identified. The sites on survivin that interact with eRF3 are located in the baculovirus IAP repeat domain (residues 65-76), which forms a beta-strand structure with an overall negative charge. The sites on eRF3 that interact with survivin were localised to the N-terminal domain(NTD; residues 131-200). Cell localisation experiments indicate that both factors are in the nucleus, suggesting that they cooperatively function in nuclear processes.

Virtual reality technology has attracted researchers’ attention because it can provide users with a virtual interactive learning environment. Based on the theory of virtual reality technology, this paper proposes the system model design and architecture of virtual interactive music-assisted interactive teaching and realizes key technologies such as modeling, music-assisted interactive teaching scene interaction, and database access. In the simulation process, based on the VRML/X3D bottom interactive system template, after comprehensive application research, comparative analysis of various modeling methods, the system verified the use of digital cameras combined with the modeling technology based on music elements to collaboratively establish VRML virtual model connections. For inline node function, we combined it with Outline3D to realize VRML integration and then use VizX3D, X3D-Edit to build X3D model and realize the conversion from VRML to X3D, which solves the system completeness problem of music-assisted interactive teaching. The experimental results show that, according to the statistical analysis of the data after the experiment, when the position changes in the virtual 3D music-assisted interactive teaching scene, it will be displayed in the plane layer, and the real-time coordinates of the virtual music-assisted interactive teaching scene displayed in HTML have case. By analyzing the scenes and dynamic effects in the works, the effects of the virtual world can be better displayed through the performance of details. The better accuracy and delay error reached 89.7% and 3.11%, respectively, which effectively improved the effect and feasibility of applying virtual reality technology to music-assisted interactive teaching.