In this paper, the microstructures of the water strider surface were observed by scanning electron microscopy. The surfaces, including wings, legs, back and abdomen, all exhibited various compound microstructures, which are the important factors influencing the superhydrophobicity with a contact angle (CA) of up to 153°. Furthermore, the grease of the water strider on different substrates was studied by self-assembly of the grease for 14 d. Images of the grease indicated that the morphology and spatial orientation of the grease depend on the substrate chosen. The grease decreased the substrate wettability by approximately 30° on highly ordered pyrolytic graphite and about 23° on the silicon substrates. Gas chromatography combined with mass spectrometry was also used to study the chemical composition of the grease layer of water strider surface. The grease layer is composed of a mixture of aliphatic compounds, which possess hydrophobicity based on the chemical structure. Grease protects the microstructures of water strider surfaces and thus results in higher CAs and hydrophobic properties. The microstructure and the grease of the water strider jointly render the hydrophobic properties of the water strider surface.