The background to the study: Allelopathy can be distinguished from other plant interference strategies because the donor species produces inhibitory compounds into the environment to cause harm. This work aimed to evaluate the allelopathic impact of aqueous leachate of Eucalyptus globulus leaf litter on seed germination, biochemical, and growth parameters of finger millet (Eleusine coracana) seedlings. Methods: This experiment was conducted using aqueous leaf litter leachate at a concentration of 100%, 80%, 60%, 40%, and 20% using water as a control. Results: The experiment showed that all the test concentrations of leachate had a deleterious effect on seed germination, seedling growth-like length, fresh and dry weight, and biochemical parameters such as total chlorophyll and total carbohydrate of the test crop, Eleusine coracana seedlings. Further, it was also observed that the inhibitory effects were gradually increased with a rise in the leaf leachate concentration. Conclusion: It is recommended that crops not be associated with different species of Eucalyptus due to the allelopathic influence on germination and early vegetative developmental stages of crops.