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In the present study carried out to investigate prophylactic activity of Cobalt nanoparticles towards isolated Escherichia coli (strain MTCC 723); the antimicrobial properties of Cobalt nanoparticles also determine by optimizing the concentration of Cobalt nanoparticles. A homogenized suspension was prepared by sonication Cobalt nanoparticles in water, and the infusion was prepared at concentrations of 5– 35µg/ml. The pathogenic strain of microbial species was cultured under anaerobic conditions. Equalized standard dilutions of the cultured bacteria were used for testing. Spectroscopic and diffusion tests were conducted to quantify the antimicrobial properties of Cobalt nanoparticles against the bacteria. Cobalt nanoparticles of 200 nm diameter with concentration of 35µg/ml showed completely lysis of Escherichia coli cell. The results showed Cobalt nanoparticles with concentration of 35 µg/ml significant higher activity against Escherichia coli. Optimized concentration of Cobalt nanoparticles kills the pathogenic Escherichia coli cells

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