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245swine serum, exhibited varying degrees of anaphylactic shock. This was characterized by dyspnea, coughing, sneezing, roughening of the coat, and prostration. A moderate to marked hyperemia and edema of the conjunctiva: also developed. Swine, rabbit, goat, and horse serum each caused approximately the same degree and type of allergic reaction in specifically sensi tized animals regardless of whether the initial, sensitizing dose had been inhaled or given parenterally. Of 56 sensitized animals which were treated in this manner, 3 died in the chamber during exposure to an atomizd specific antigen. Two of these had been sensitized by a single intraperitoneal injection of 0.3% crystalline egg albumin, the third by inhalation of the same substance. Other than this, however, anaphylactic reactions were sub-lethal and symptoms usually subsided within a few hours after each exposure to aerosol. Control animals gave slight or no reactions. Four weeks after sensitization by inhalation of various antigens, 14 guinea pigs were injected intravenously with 0.3 to 0.5 cc of the specific antigen. Twelve of the 14 animals in this group died of typical anaphylactic shock. I t has been suggested that repeated inhalations of small doses of anti-influenza1 Perum might be used prophylactically. A second series of experiments, using goat serum alone, was conducted to determine whether or not widely spaced inhalations of smaller quantities of serum would also produce a state of zctive hypersensitivity. Guinea pigs and rabbits were exposed to aerosols of undiluted or diluted (1:IO) goat serum for 20 minutes at weekly intervals. Upon the third of the weekly exposures, mild reactions were observed. Ry the fifth week of such treatment, allergic reactions were quite marked and were in every way similar to those previously described.Histologic studies of tissues from animals which were autopsied two days following the last of several ( 5 or 6 ) 20-minute expusures to atomized antigen revealed acute inflammatory changes in lungs, trachea, and nasal mucosae. These were present to some extent in all of the animals, including controls, but were more pronounced in those which had been previously sensitized by injection or inhalation of the specific antigen.From these observations it is apparent that active sensitization can be readily accomplished by inhalation of finely atomized fluid antigen and that serious allergic reactions, even fatal anaphylactic shock, may occur when hypersensitive animals inhale aerosol of specific antigen. Experimental Basis for the Chemotherapy of Trichomonas vaginalisInfestations. I.

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This study is a continuation of a survey of the physiologic activities of a bacteria-free culture of Trichon.zoizns ~aginalis.l-~ Knowledge of the fermentation reactions of this organism is desirable to serve as a basis for the comparison of this s p i e s with other pure cultures when they may become available. Moreover, since compounds containing carbohydrates are extensively used in the therapy of vaginal trichomoniasis, the demonstration of any trichomonasgrowth-stimulating action of a carbohydrate suggests that it would probably be of limited clinical value.All experiments were per formed in duplicate and for those compounds utilized by the protozoa a third test was made.Utilization of the various substances was determined by comparing the population increax and pH shift in a basic medium with and without the test compounds. Four tubes with the test compound were used in most of the experiments and the results averaged. Population counts were made in hemocytometers (one cubic mm counted per tube). pH measurements were made with glass electrode equipment standardized each time against 2 known buffers.The basic medium employed and recommended, which gives very poor growth in the absence of utilizable carbohydrates, is prepared as follows:Two percent proteose peptone No. 3 (Difco), 0.5% NaCl, and 0.1% agar in distilled water adjusted to pH 6, is tubed in 9 cc amounts and autoclaved. After cooling, 0.5 cc of sterile filtered undiluted human serum (adjusted to pH 6 with N/l HC1) and sufficient autoclaved concentrated sodium thioglycollate solution to give a final concentration of 0.1 % are added. The latter is neces-Experimeiztnl.