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The synthesis of DNA products complementary to artificial templates by the enzyme RNA-directed DNA polymerase isolated from avian myeloblastosis virus has been studied. Of the single base polyribonucleotides, poly(rC), poly(rA), and poly(rI) were active while poly(rG) and poly(rU) were almost inactive. The minimum length showing activity for an oligo(rC) template was 9; the minimum primer length of oligo(dG) was 3 or 4. In order to examine the fidelity of transcription, single base oligoribonucleotides of defined length were studied. Using (rC)1 as template and (dG)8 as primer, the oligo(dG) product coelectrophoresed w th the template. However, using (rA)0 as template and (dT) as primer, a large (10-16S) product was formed.

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