Fourteen tetraploid seedling populations of blackberry (Rubus sp.), representing quadruplex, (TTTT), triplex (TTTt), duplex (TTtt), simplex (Tttt), and nulliplex (tttt) genotypes for the major gene conferring thorniness, were evaluated for segregation of cane thorn density and cotyledonary gland number. Comparisons of seedling distribution curves, means and variances of segregating and non-segregating populations did not show a gene dose effect on gland and thorn occurrence. Inheritance of cotyledonary glands and cane thorns in blackberry was qualitative with the density of glands and thorns apparently controlled by several modifying genes.
This experiment was conducted in a nonbearing 3-year-old ‘bluecrop’ blueberry field, near Hammonton, NJ. Soils at this location were sandy loam with 4.7 pH and irrigated season long. Blueberry bushes were approximately 3-4 ft tall and spaced 3 × 8 ft apart. Treatments were replicated 6 times in a randomized complete block design with each replicate consisting of 48-ft-long rows with 10-12 bushes in 2 to 4 rows. Treatments were separated by at least 2 unsprayed rows and 4 unsprayed plants within a row that served as buffer. Insecticides were applied as a 1-ft band on both sides of the plant for a total of 96 sq ft of treated area per replication. Granular insecticides were applied by spreading the granules evenly by hand and incorporated to a depth of 1-2 inches using a hand hoe. Liquid formulations were applied with a CO2 pressurized Model T backpack sprayer (R&D Sprayers) equipped with a single TeeJet nozzle (8002VS) operated at 35 psi. Insecticides were applied twice, on 3 Jun and 24 Aug. Insecticides were sprayed using 1.5 liters of water per replicate on 3 Jun and 1 liter on 24 Aug (30—45 gal/acre). Following each application the field was irrigated by drip irrigation with at least 1 inch of water. Treatment effects were evaluated by examining soil and roots in 1-ft-diameter by 1-ft deep areas around the plants (2 plants/replicate) for whitegrubs (including OB, JB, AGB) and mealybugs. Colonies of newly hatched nymphs of mealybugs were counted separately. Plants were sampled between 20-27 Jun following the first application and between 12-19 Sep following the second application.
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