Radiolabeled hexadecyl cholesteryl ether can serve as an effective marker for liposomes in a variety of studies. This paper demonstrates the use of a cholesteryl ether marker in the assay of phospholipid transfer protein activity and in assessing phospholipid vesicle‐cell interactions. The cholesteryl ether has the advantages of ease of synthesis, metabolic inertness, lipid solubility and nonexchangeability.

The fluorescence of cattle rod outer segments (dried) and of rhodopsin in solution lies in the range of 575 to 600 millimicrons with a quantum efficiency of 0.005 if excitation is in the visible band near 500 millimicrons. The emission is abolished by bleaching at -196 degrees C but can be reversibly regenerated by irradiation with light of longer wavelength (600 millimicrons). This behavior reflects the known interconversion of rhodopsin to prelumirhodopsin at this temperature.

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