Introduction: Timely diagnosis of congenital heart disease (CHD) is challenging but critical. Children with such life-threatening defects may not initially have symptoms or the symptoms may be vague, and the condition is not detected on routine clinical examination in the majority of cases. In the UK alone, up to 100 otherwise healthy infants may be dying every year from undiagnosed CHD. Pulse oximetry is the rst, appropriately simple method to have been used for universal screening of CHD, and the earliest reports (abstracts) on pulse oximetry screening were published in 1995. Aims: To assess the importance of pluse oximetry as a screening tool in diagnosis of congenital heart disease in newborn. Materials And Methods:The study was conducted in postnatal ward of BMCH from Preparatory phase-1st september2018 to 15th October 2018, Data collection-1st January 2019 to 31th December 2019 and Data analysis phase- 1st January 2020 to 30th April 2020. 500 babies who are randomly selected from total study material. Result: Our study showed that 6(1.2%) patients were Abnormal, 475(95.0%) patients had ND and 19(3.8%) patient were Normal. 6(1.2%) patients were Abnormal, 475(95.0%) patients had ND and 19(3.8%) patient were Normal. 20(4.0%) patients were Abnormal and 480(96.0%) patient were Normal. 20(4.0%) patients were Abnormal and 4(0.8%) patients were Normal. 6(1.2%) patients had only pphn and 2(0.4%) patients had pphn. 2(0.4%) patients were Abnormal. 495(99.0%) patients had Acyanotic heart disease and 5(1.0%) patients had Cyanotic heart disease. Conclusion: We found that 2(0.4%) patients were Abnormal ANC, 4(0.8%) patients were RR Abnormal and 5(1.0%) patients had cyanosis, it was found that 16(3.2%) patients had Murmur and 6(1.2%) patients were abnormal chest X-Ray and we found that 6(1.2%) patients were Abnormal ECG and 20(4.0%) patients were Abnormal ECHO.