Aims:  To validate the effectiveness of a miniaturized most probable number method (mMPN) in enumerating Salmonella from poultry matrices. Methods and Results:  A MPN was developed, based on the ISO 6579:2002 method using modified semi‐solid Rappaport–Vassiliadis media as the sole selective medium. The validation of the mMPN was shown to not differ significantly from, at the 95% confidence level (Student’s t‐test P = 0·357) to, the traditional 9‐tube MPN (tMPN) using pure cultures of Salmonella ser. Typhimurium, Infantis, Montevideo, Muenster and Salmonella subsp II 1,4,12,27:b:[e,n,x] (Sofia). The validation of naturally and artificially contaminated poultry matrices (carcasses, scald tank water, faeces, caeca and feed) showed that detection using the mMPN compared well to the ISO 6572:2002; sensitivity (92%), specificity (97%) and agreement (KAPPA 0·72). The quantitative comparison between the tMPN and mMPN methods showed that 92% of enumerations were less than ±1 log different (Student’s t‐test = 0·13). Financial analysis showed that the mMPN required 64% less media and 56% less labour than the tMPN. Conclusion:  The mMPN is a consistent, easy to automate method for the enumeration of Salmonella from different poultry matrices. Significance and Impact of Study:  The miniaturized MPN reduces the material and labour cost of the method and enables the uniform and accurate measurement of the effectiveness of intervention strategies in the control of Salmonella colonization of poultry.

Salmonella contamination of ground beef has been viewed as originating from the surface of carcasses. Recent studies have identified lymph nodes as a potential source of Salmonella contamination because these tissues play an active role in containment of pathogens in the live animal and because some lymph nodes are unavoidably present in manufacturing beef trimmings or primal cuts that may be incorporated into ground beef. A survey was conducted of the microbiological status of lymph nodes from Australian cattle at the time of slaughter to determine the prevalence of microbiological contamination. Sets of lymph nodes (n = 197), consisting of the superficial cervical (prescapular), prepectoral, axillary, presternal, popliteal, ischiatic, subiliac (precrural), coxalis, and iliofemoralis (deep inguinal), were collected from five geographically separated Australian abattoirs over a period of 14 months. Samples were tested for the presence of Salmonella spp. and Shiga toxin-producing Escherichia coli by BAX PCR assay. Aerobic plate count, E. coli, and coliforms were enumerated with a lower limit of detection of 80 CFU per node. The observed prevalence of Salmonella within peripheral lymph nodes was 0.48% (7 of 1,464). Two of the seven lymph nodes in which Salmonella organisms were detected came from the same animal. Grass-fed, grain-fed, and cull dairy cattle were all found to have detectable Salmonella in lymph nodes. All Salmonella detections occurred during cooler months of the year. No Shiga toxin-producing E. coli were detected. Aerobic microorganisms were detected above the limit of quantification in 3.2% of nodes (median count 2.24 log per node), and E. coli was detected in 0.8% of nodes (median count 3.05 log per node). The low prevalence of Salmonella and low concentration of aerobic microorganisms in Salmonella-positive lymph nodes of Australian cattle at the time of slaughter suggest that the likelihood of lymph nodes contributing significantly to the presence of Salmonella in ground beef is low.