Cryptosporidium an apicomplexan parasite has the ability to induce diarrhea in bovines, goats, pigs, dogs and cats worldwide. In this study, buffalo calves fecal samples were examined after staining their smears with Modified Ziehl-Neelsen Stain (MZN). Ileal sections were examined for the detection of pathological changes. Further molecular characterization was done using nested PCR amplification and partial sequence analysis. The detected oocysts were morphologically similar to Cryptosporidium parvum. Light microscopic examination of Cryptosporidium infected ileal Tissue Section (TS) stained with H and E revealed the presence of altered mucosal architecture with congestion of blood vessels, infiltration, sloughing and complete erosion of epithelial cells and shortening, blunting, stunting and atrophy of the intestinal villi. Molecular characterization gave PCR amplicons of 18S SSU rRNA gene products approximately at 823 bp. Sequences proved specified generalized relatedness with 21 species of Cryptosporidium but the nucleotide homogeneity percentage was insufficient to designate species or genotypes. Further bioinformatics analysis showed that resulting Cryptosporidium isolates had the closest match with three isolates. It was implied that the Cryptosporidium isolates is mostly like Cryptosporidium parvum (JX237832.1) previously isolated from buffaloes in Ismailia province.
T Temiurus shalabyi sp. n. is the first record of the genus from Lake-£ JL Timsah and the second species of the genus in Egypt It is described from the stomach of Trachurus indicus (Carangidae) locally named "Bagha Om Ain" from Lake Timsah. The prevalence of the parasite was 18.8% with intensity of infection being 2-4 worms per fish. Hemiurus shalabyi is easily distinguished from all the other known species of the genus by the number, shape and position of vitellaria, in addition to the extension of the intestinal caeca and the uterus into the ecsoma. The differences between the present new species and the previously described species of the genus from Egypt, K suezensis Aboul-Hag, 1990 were discussed.
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