Summary
The basic aim of the study was to utilise Himalayan Walnut oil (HWO) encapsulated in soy protein isolate (SPI)–maltodextrin (MD)–pectin (Pec) complex as a potential source of omega fatty acids for bread fortification. Encapsulated HWO was integrated with bread for enhanced quality and technological aspects compared to control: it contained protein (9–19%), fat (6–8%), alpha‐linolenic acid (37.50%) with lower carbohydrate (35–42%) content. The baked loaves depicted higher specific loaf volume (3.85–4.85 cm3 g−1) with low hardness (4.24–5 N) due to the presence of hydrocolloids. Crust and crumb colour revealed a significant effect on the appearance of bread, with an increase in the antioxidant activity as assessed by 1,1‐diphenyl‐2‐picrylhydrazyl (80%), 2,2′‐Azino‐bis (3‐Ethylbenzothiazoline‐6‐Sulfonic Acid) (100%) and Metal Chelating Activity (10%). Compared to control, addition of encapsulated HWO increased final product quality by lowering oil oxidation during storage period. Scanning electron microscopic analysis showed intact encapsulated oil bodies in crumb after baking, thereby demonstrating high omega fatty acid retention.
The basic aim of this study was to encapsulate Himalayan
walnut
oil (HWO) in different concentrations of soy protein isolate (SPI),
maltodextrin (MD), and pectin at varying pH (4.0 and 7.0) by complex
coacervation. The formation of primary and secondary oxidation products
showed a 6–8-fold increase in free oil (0.8–60 meq O2/kg, 1–88) compared to encapsulated HWO (1–40
meq O2/kg, 1–34) for a storage period of 60 days.
The coacervates developed between SPI–MD–pectin at pH
4.0 exhibited favorable physicochemical properties like an optimum
moisture content, a
w, color, and density
with maximum retention of omega fatty acids (69%). The antioxidant
potential assessed by 2,2′-diphenyl-1-picrylhydrazyl (DPPH*)
and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
(ABTS*) and the metal chelating ability (MCA) showed that coacervates
prepared at pH 4.0 depicted a lower DPPH [142%] and a higher ABTS
[39%] and MCA [41%] than those at pH 7.0 (DPPH [144%], ABTS [34%],
and MCA [38%]). An in vitro digestion model showed increased antioxidative
properties after gastric digestion compared to intestinal digestion.
The amount of free fatty acids released from gastric digested emulsions
during gastrointestinal digestion was 1400 mg/g with maximum omega
fatty acid retention. The model indicated good lipid digestion and
absorption of lipophilic bioactives in the small intestines.
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