Chemical oxygen demand to nitrogen (COD/N) and nitrogen to phosphorus (N/P) ratios have distinct effects on bacterial community structure and interactions. However, how organic to nutrient imbalances affect the structure of freshwater bacterial assemblages in restored wetlands remains poorly understood. Here, the composition and dominant taxa of bacterial assemblages in four wetlands [low COD/N and high N/P (LH), low COD/N and low N/P (LL), high COD/N and high N/P (HH), and high COD/N and low N/P (HL)] were investigated. A total of 7,709 operational taxonomic units were identified by high throughput sequencing, and Actinobacteria, Proteobacteria, and Cyanobacteria were the most abundant phyla in the restored wetlands. High COD/N significantly increased bacterial diversity and was negatively correlated with N/P (R2 = 0.128; p = 0.039), and the observed richness (Sobs) indices ranged from 860.77 to 1314.66. The corresponding Chao1 and phylogenetic diversity (PD) values ranged from 1533.42 to 2524.56 and 127.95 to 184.63. Bacterial beta diversity was negatively related to COD/N (R2 = 0.258; p < 0.001). The distribution of bacterial assemblages was mostly driven by variations in ammonia nitrogen (NH4+-N, p < 0.01) and electrical conductivity (EC, p < 0.01), which collectively explained more than 80% of the variation in bacterial assemblages. However, the dominant taxa Proteobacteria, Firmicutes, Cyanobacteria, Bacteroidetes, Verrucomicrobia, Planctomycetes, Chloroflexi, and Deinococcus-Thermus were obviously affected by variation in COD/N and N/P (p < 0.05). The highest node and edge numbers and average degree were observed in the LH group. The co-occurrence networkindicated that LH promoted bacterial network compactness and bacterial interaction consolidation. The relationships between organic to nutrient imbalances and bacterial assemblages may provide a theoretical basis for the empirical management of wetland ecosystems.

The effects of coastal embankments on nitrogen (N) cycling in the Spartina alterniflora salt marsh have been extensively reported. However, it remains unclear effects of the embankment on the sizes of diverse N subpools in the plant-soil subsystems year-round. This study examined seasonal changes in various N subpools of plant subsystems; soil subsystems [e.g., soil organic N (SON), recalcitrant organic N (RON), labile organic N (LON), dissolved organic N (DON), ammonium N(NH+4 N), and nitrate N(NO3-N)]; N mineralization [e.g., soil net ammonification (RA) and nitrification (RN) rate]; and immobilization [e.g., microbial biomass N (MBN)] in embanked and adjacent S. alterniflora natural salt marshes on the coast of Eastern China. The embankment significantly reduced the litter N storage by 62.7–71.8% over the four seasons and decreased the root N storage by 53.0% during winter. The SON, LON, RON, and N H +4 N concentrations declined significantly by 43.0–60.2%, 35.8–64.8%, 44.9–59.0%, and 20.8–42.2%, respectively, over the four seasons following the embankment construction. Furthermore, the embankment dramatically reduced the DON concentrations by 21.9% in spring, 14.6% in summer, and 10.4% in winter, while notably diminishing the NO3 N concentrations by 33.4% in autumn and 44.9% in winter, and the RA and RN in spring and summer. However, the embankment clearly increased the MBN concentrations during summer and autumn, the NO3 N concentrations in spring, and the RA and RN in winter at different levels. Due to the decreased soil N inputs from plants, the embankment decreased the organic and inorganic N subpools every season to varying degrees, except for the NO3 N concentration in spring. We suggest that the decreased soil salinity following embankment establishment might increase the uptake of ions by microbes, while stimulating the production of MBN. Ultimately, the NO3 N and DON were two vital N sources for S. alterniflora, and plants absorbed N from the soil to promote their biomass, as well as N concentration and storage. This study is conducive toward understanding the mechanisms behind the effects of coastal embankments on the N transfer among various N subpools in the plant and soil systems.