EU legislation restricted many chemicals for root-knot nematodes (Meloidogyne spp., RKNs) control. Alternative ways of plant protection were hence investigated, based on the use of microbial formulations exploiting plant parasites antagonism. RKNs are severe and widespread pests causing extensive damage to crops in greenhouse and field. Several antagonistic microorganisms are suitable for biocontrol, including the nematophagous hyphomycete Pochonia chlamydosporia that parasitizes eggs to acquire additional nourishment and face competition with other soil microorganisms. A commercial product (POCHAR) was developed by Microspore based on P. chlamydosporia with other microbial inocula that can be applied through irrigation. The aim of this study was to test POCHAR's efficacy against RKNs on potato and its promotion effect on tomato in two field trials. Moreover, the research included trials to evaluate the best method for open-field application in crops not managed through drip irrigation, opening up the possibility to treat large areas without major technological needs. RKNs control with POCHAR represented a viable alternative to chemicals. In conclusion, the organic approach developed through the bioformulated product highlighted effective RKNs management, with a potential to sustain both plant nutrition and the related root protection needs. ARTICLE HISTORY

Abstract:The assessment study of the global ecological impact tries to highlight the main factors and negative ecological determinants, due to a lack or excess, and it also focuses on highlighting the main negative ecological effects with the aim to rehabilitate and restore the ecological balance within degraded ecosystems. The methodology used in the assessment process was based on graphs, tables in the shape of Leopold matrix, considerably improved by authors. In order to assess qualitatively the negative ecological effects, a reliability scale with 3 indicators and 3 graduations was used, designed to underline the importance of the impact (minor, medium, and major), the quality of the impact (neutral, negative, and positive) and the certainty of the appearance of a negative impact (improbable, probable, and certain). Our research was accomplished in the pasture ecosystem degraded by pluvial erosion from the Tutova Hills, located in the Eastern part of Romania. This ecosystem is characterized through active geo-morphological processes in the depth and on the surface and it drew attention to the presence of 8 negative ecological factors grouped in 3 main categories: geo-climatic, pedological, and anthropogenic. 8 main negative effects were identified and quantified by means of 3 qualitative indicators with 3 graduation scales. The analysis of the current state of the effects of the complex ecological impact upon the degraded ecosystem ask for a series of urgent measures elaborated by scientists, researchers, and representatives of the local administration system. The aim of these measures is to improve the ecological balance and to eliminate the negative anthropogenic impact that augments and aggravates the action of the negative geo-climatic and pedological factors, in of with the protection of soil quality.

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Currently there is a growing interest both in obtaining large agricultural yields in order to provide food for a growing population as well as in increasing the sustainability of production systems by improving the health of the soil. The overall objective of this study was to evaluate the effect of organic versus chemical fertilization versus irrigation on runner bean crop and soil health. A bi-factorial field experiment was organized in a split plot design with three replicates during two years (2019 and 2020). The first factor, irrigation, had two graduations: irrigated with 3000 m 3 ·ha-1 (Ir) and non-irrigated (Non-Ir). The second factor, fertilization, had four graduations: biosolids (1200 kg·ha-1 ) (B), chemical (360 kg·ha-1 ) (Ch), microorganism (80 kg·ha-1 ) (M) and unfertilized (Unfert). The results showed that, regarding the physiological state of the plants, both the photosynthesis and the chlorophyll content were higher at Ir x M (5.31 µmol CO2 m -2 s -2 and 45.23 SPAD units, respectively). The yield of dried beans ranged between 1476-4811 kg·ha-1 in 2019 and 1498-4727 kg·ha-1 in 2020 and was highest for Ir x Ch. Soil health status estimated by determining the dehydrogenase activity in the soil, has been improved for irrigated (Ir) and fertilized (B, M, Ch) variants.