B.binotatus is native fish from Ciliwung River whose existence is decreasing due to the similarity of food sources between native species and introduced species, causing competition to seek food. The study aimed to determine the trophic level and position B. binotatus in the Cilliwung River ecosystem based on gut content analysis using the purposive sampling method during August-October 2021. Data were obtained from a total of 60 samples from the Ciesek River, Cirangrang, and Sukabirus. The fishes were dissected, and gut contents were observed using a light microscope, with observations repeated 3 times for each sample. Data analysis includes the relative length of gut, index of preponderance, nichce area, niche overlap, and trophic level of an organism. The feed source consists of Bacillarophyta (68.62%), Chlorophyta (16.40%), Manogononta (8.41%), Cyanophyta (2.81%), Euglenaphyta (1.70%), Amoebozoa (1.01%), Copepods (0.93%), and detritus (0.11%). The result showed that B. binotatus in Ciliwung River is at trophic level II and included as an omnivore (2.1 < Troph < 2.9). B. binotatus is as an omnivore and have a common food source with introduced species, causing competition to seek food.