A pesquisa científica é o alicerce para a evolução do conhecimento humano. Este estudo tem como objetivo descrever a experiência acadêmica na iniciação científica por meio da pesquisa experimental in vivo. A iniciação científica foi desenvolvida com o uso de terapias alternativas no tratamento da cicatrização da pele em ratos. O público-alvo envolveu alunos dos cursos de bacharelado em Enfermagem, Farmácia e Biologia. Durante a iniciação científica, os alunos participaram da leitura crítica de artigos científicos, da redação do projeto de pesquisa, do desenvolvimento experimental, de protocolos experimentais e de eventos científicos. A experiência resultou para o aluno no avanço na fronteira do conhecimento sobre as ciências básicas e no uso de modelos animais na pesquisa experimental. Contudo, a obtenção de recursos financeiros e humanos representou um obstáculo durante a condução das atividades. Conclui-se que a iniciação científica agrega valores essenciais na formação acadêmica, principalmente no que se refere à capacidade de tomada de decisão e de raciocínio crítico. Além disso, para o contexto deste relato resultou no maior interesse e na procura pela pesquisa científica.
Fluoxetine is an emerging pollutant that acts as a selective serotonin reuptake inhibitor (SSRI) and being a hydrolytic molecule that is photolytically stable and accumulaties in biological tissues, its disposal in the aquatic environment can interfere with the physiology of fish and shrimp. Thus, the objective of this study was to analyze the effects of fluoxetine on routine metabolism (metabolic rate, specific ammonia excretion and O:N ratio) of Deuterodon iguape and Palaemon pandaliformis. For this, five groups of each species, were exposed to different concentrations of fluoxetine for 24 hours (D. iguape) and 2 hours (P. pandaliformis). The results demonstrated that in D. iguape exposure to fluoxetine significantly increased both the metabolic rate by 75%, 85%, 55% and 50% for concentrations of 0.05; 0.1; 0.5 and 1.0 mgL -1 , respectively, and the specific ammonia excretion by 40%, 48% and 20% for concentrations of 0.05; 0.1 and 0.5 mgL -1 , respectively, when compared with their control. The O:N ratio was statistically greater in concentrations of 0.5 and 1.0 mgL -1 . Concerning P. pandaliformis, exposure to fluoxetine increased metabolic rate at concentrations 30.0 and HIGHLIGHTS Fluoxetine increases the metabolic rate and excretion of ammonia in both species. O:N ratio in fish showed higher values in the highest concentrations of fluoxetine. The LC50 -96 hour values of Palaemon pandaliformis represented greater toxicity. Both species are a good biological model for fluoxetine exposure studies. Rezende, K.F.O.; et al.
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