The study of insect fauna and their development and succession patterns on decaying cadavers is crucial to promoting insect evidence as a useful tool in forensic science, particularly for estimating the postmortem interval (PMI). Body decomposition and arthropod succession are affected by many factors and exhibit substantial regional variations; therefore, detailed succession studies in different biogeographic regions are required for understanding the successional patterns of insects in various environments. This study was conducted in the summer of 2021 using three domestic pig carcasses (Sus scrofa domestica L., 1758) in the field of Shizuishan City, Ningxia, Northwest China. A total of 40 species of necrophagous insects belonging to three orders and 16 families were collected. Among Diptera, Lucilia sericata (Meigen,1826), Chrysomya megacephala (Fabricius,1794), and Phormia regina (Meigen,1826) (Diptera: Calliphoridae) were the dominant species. As for Coleoptera, the dominant species changed throughout the process of carcass decomposition from Saprinus semipunctatus (Fabricius,1792) (Coleoptera: Histeridae) to Dermestes maculatus DeGeer,1774 and Dermestes frischii Kugelann,1792 (Coleoptera: Dermestidae). The carcasses desiccated rapidly and reached the remains stage under extreme conditions of high temperatures and low humidity, after which a large amount of dried tissue of the carcasses attracted populations of Coleoptera, particularly Dermstidae, which were abundant and remained until the end of the experiment on day 50. The current study is the first forensic entomological investigation of succession in Northwest China and provides basic data for the estimation of PMI during summer in this region.

Necrobia ruficollis (Fabricius, 1775) (Coleoptera: Cleridae) is an important cosmopolitan storage pest, and also frequently appears on highly decomposed and skeletonized corpses. It is a forensically important species expected to indicate a longer postmortem interval (PMI). Therefore, we investigated the development of N. ruficollis at five constant temperatures between 22 °C and 34 °C. Under temperatures of 22, 25, 28, 31, and 34 °C, the mean (±SD) developmental durations from eggs to adults were 93.00 ± 1.63, 70.67 ± 0.94, 65.33 ± 3.40, 47.33 ± 0.94, and 56.66 ± 8.73 days, respectively. According to the developmental time and accumulated degree hours results, an isomorphen diagram and thermal summation model were generated. The calculated values of developmental threshold temperature and accumulated temperature constant were estimated by a linear model to be 14.51 ± 0.52 °C and 684.12 ± 33.85 degree days, respectively. Lower developmental thresholds, intrinsic optimum temperature, and upper lethal developmental threshold temperature were estimated by a nonlinear model to be 14.61, 25.90, and 34.94 °C. Morphological indexes of larvae were obtained by in vivo measurements. A growth curve and an equation of the relationship between development time and body length were simulated. In addition, the widths of the head capsules and the distance between the urogomphi of larvae at different instars were determined by cluster analysis. Classifiers were created and validated by linear discriminant analysis. These results provide important basic developmental data for using N. ruficollis to estimate the minimum postmortem interval (minimum PMI). However, this study was only conducted under constant temperature, and the applicability of these data to variable temperature conditions needs to be further confirmed.

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