The effect of irradiation on microbial load and shelf-life of anchovies (Engraulis encrasicolus) was assessed in this study. Irradiation doses used in the study were 2.5, 5.0, 7.5 and 10 kGy. The assessment was carried out at 3 weeks intervals for the period of 9 weeks. Samples were analyzed for microbial load (total viable count, total coliform count and Staphylococcus aureus). Smoked samples were more contaminated as compared to sun-dried samples from both locations. A dose dependent effect was observed in all the samples obtained from both Chorkor and Keta (p<0.05). Samples from Keta were less contaminated for both smoked and sundried samples as compared to the samples from Chorkor whether sun dried or smoked (p<0.05). At 2.5 kGy, microbial load levels (CFU/g) for total viable count, total coliform count and S. aureus were below the standard (CFU/g of microbial load) set by the Ghana Standards Authority for microbial load in fish. This dose is the most appropriate dose for the decontamination and shelf extension of anchovy from Ghana.
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