This case report describes the intralesional application of autologous conditioned plasma (ACP) in seven horses as treatment of severe tendinitis of the superficial digital flexor tendon, deep digital flexor tendon, or desmitis of the inferior check ligament. Follow-up data of the horses revealed a positive outcome in 10 to 13 months post injury. All horses treated with ACP were either performing in their previous work-load or were back in full training. Further studies with long-term follow-up will have to be performed to support these clinical intermediate-term observations.
There has been insufficient research into CO2 stunning with regard to its effect on pigs being slaughtered. This lack of knowledge may be at least partly responsible for the partial rejection of CO2-stunning methods. During routine slaughter work, 598 pigs (average carcase weight: 94 kg) were evaluated. The stunning procedure was carried out in industrial stunning chambers with 90% CO2 by volume and an exposure time of either 120 or 90 s. The corneal reflex response was evaluated immediately prior to bleeding in order to determine the depth of narcosis. Blood was taken at slaughter (slaughter blood) to determine the partial pressure of breathing gases and the acid-base status. We found that CO2 stunning mainly produced hypoxaemia, but also normoand hyperoxaemia, in arteriovenous slaughter blood. No further positive reflex responses occurred at a pO2 threshold of ≤ 1.6 kPa. PCO2 increased to values of 40 kPa and above. This extreme hypercapnia resulted in a decrease of the slaughter blood pH with values of less than 7.00 (ie, strong respiratory acidosis). Starting with threshold values from pCO2 > 23 kPa and pH < 6.85, stunned pigs revealed only a few or no positive reflex responses, respectively. The non-respiratory Stewart-variable serum [SID3] was elevated to alkaline values of 65 mmol L−1 and above, in comparison to the normal values of 45 (± 2) mmol L−1. We conclude that the use of cut-off points such as the pH and/or pO2 in routine sampling of slaughter animals (eg by application of ion-sensitive electrodes) would establish the depth of narcosis in pigs destined for slaughter. The efficiency of monitoring could thereby be improved during slaughter, in line with the demands of animal welfare.
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