The yeast Candida bombicola ATCC 22214 is well-known to produce mixtures of glycolipids containing the sugar sophorose, the so-called sophorolipids, especially when cultivated on hydrophobic carbon sources as co-substrates. To improve cultivation efficiency, an integrated process was developed using ultrasound separation technology. Since this technology is new for use with C. bombicola, it was first characterized in batch experiments and afterwards implemented in an integrated production process. In this process, separation efficiencies of about 99% C. bombicola cells could be achieved, leading to 8 g/L of nearly cell-free sophorolipid product and a total amount of 73.8 g/L sophorolipids. Furthermore, a technical mixture of unusual branched fatty alcohols containing mainly 2-hexyl-1-decanol was used as co-substrate with glucose in a shake flask study. This resulted in the production of a new product, 1-O-b-glucopyranosyl-2-hexyldecanol, a molecule containing glucose as the sugar moiety and 2-hexyl-1-decanol as a branched hydrophobic side chain.