undulant fever sixteen months ago, a strain correspond¬ ing to Brucella abortus (porcine) has been isolated. These organisms are apparently identical with those obtained from the blood culture early in his illness.In 1911 we7 observed that B. dysenteriae agglu¬ tinated by specific serum would yield good growth even after several hours' standing. This fact was applied to the isolation of Brucella from stools. METHOD About 1 Gtn. of fresh feces was mixed in 50 cc. of sterile isotouic salt solution and shaken for a few minutes to insure thorough suspension. The suspen¬ sion was filtered through four layers of number 1 hos¬ pital gauze to remove gross particles and centrifugated at half speed for three minutes to thrown down other particles and larger bacteria. To the supernatant sus¬ pension, a sufficient amount of immune serum was added to make the total dilution 1: 100, and, after shaking, the mixture was placed in a 37 C. water bath for two hours. The suspension was centrifugated at half speed for five minutes and the supernatant fluid discarded.The precipitate was resuspended in isotonic salt solution, stirred, and centrifugated at the same speed again. The supernatant fluid was again discarded and the procedure repeated twice. Finally the precipitate was spread with a bent glass rod on eosin-methylene blue plates, some of which were incubated at 37 C. aerobically and others in an anaerobic jar containing 10 per cent carbon dioxide.
Dr. Perlstein presents the problem in the care of cerebral palsy patients as it confronts us today. The monograph is concise, well written, and reveals the author's comprehensive knowledge of the subject.
Part one is concerned with a general description of the problem and discusses the causes, types, prognosis, incidence and other pertinent information and observations. Our present facilities for care are outlined, and this picture is a sorry one indeed. Only about 10% of these unfortunate individuals receive adequate care at the present time.
Part two offers a well thought out and sensible plan to provide effective care. The proposed In-Patient Hospital-Schools and Out-Patient Clinic-Schools administered at state levels seem to offer a method of care far superior to any plan existing in most of our states today. There are a few states which have already inaugurated this or a similar plan.
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