No abstract

In 30-min free-operant tests, the dopamine receptor blockers pimozide (.125, .25, and .50 mg/kg) and (-f-)-butaclamol (.1, .2, and .4 mg/kg) attenuated lever pressing for lateral hypothalamic brain stimulation. When discrete self-stimulation trials were offered in a straight alleyway, pimozide increased start box latencies, slowed running speeds, and reduced lever-pressing rates. However, performance early in both lever-pressing and runway sessions was normal; performance deteriorated as testing progressed, following patterns that paralleled those seen when animals were tested with reductions in the amplitude of stimulating current. Spontaneous recovery was obtained in both situations; experimenter-imposed 10-min time-outs caused renewed lever pressing and running. In contrast, a-noradrenergic receptor blockade by phenoxybenzamine (5,10, and 20 mg/kg) failed to produce extinction-like response patterns. These data support the view that central dopaminergic systems are important components of the neural mechanisms mediating reward.

The chronic mild stress (CMS) procedure was developed in rodents to target anhedonia, the core symptom of depressive melancholia. Stress exposure has been shown to induce a variety of physiological, biochemical and behavioral alterations associated with depression, although its anhedonic consequences as indexed by either sucrose intake and preference or thresholds for brain stimulation reward are less reliably observed. In the present study, we assessed the effects of six weeks of CMS on the latter measure in two strains of male and female rats subsequently challenged with an acute psychophysical stressor, forced swimming; their behavior in the swimming cylinder was evaluated on two consecutive days. While brain stimulation reward thresholds and response rates were unchanged by CMS exposure, significant differences in forced swim behaviors were observed between male control and CMS groups. In particular, male Long Evans rats with a history of CMS showed the largest decrease in the duration of active behaviors on the second test day, a pattern less evident in the Sprague-Dawley strain of rats, or in any of the female groups. The results suggest that the effects of depressogenic manipulations are strain and gender dependent, with male Long Evans rats most susceptible, as demonstrated by the selective reduction of struggling behaviors. Inclusion of multiple measures, including the forced swim test, would provide a better understanding of the psychopathological profile engendered by chronic exposure to mild stressors and its genetic specificity.

To identify variables that underlie intuitive judgments about the sizes of groups of similar objects, we asked people to judge the relative heights of vertical bars briefly shown, two groups at a time, on a computer display. Randomly selected normal deviates determined individual bar height. Average differences in height and group sizes were also randomly varied. Twenty-eight participants judged 250 differences each, which were then submitted to multiple regression analysis and psychophysical inspection. The total number of bars sharpened discrimination, whereas variance dulled it. Critical ratio (CR), the forerunner to the modern t test, emerged as the most important predictor; little additional variance was explained by other factors. The difference in the number of bars was a reliable factor, favoring the greater number of bars. Confidence limits around thresholds, defined as CRs needed to say "possibly greater," surrounded 1.65; as a z value, this corresponded to a one-tailed probability of .05. Judgments about noisy stimuli thus seem to be based on a statistical process and to employ a probability criterion similar to that used in the formal statistical evaluation of experimental findings-namely, p .05.

A novel approach to estimating the current density required to directly activate axons involved in the reinforcing effect of brain stimulation is described. Self-stimulating rats received trains of cathodal pulse pairs via two adjacent, stimulating electrodes which were positioned in the lateral hypothalamus and oriented to lie in a plane transverse to the medial forebrain bundle. The first pulse of each pair was delivered through one electrode and, after varied delays, the second was applied to the other electrode in an attempt to detect a loss of stimulation effectiveness attributable to refractoriness of axons that penetrated the intersection of the two stimulation fields. In low-current tests, no change in the psychophysically scaled effectiveness was observed as the interval separating the pulses was varied but, at higher currents, the stimulation effectiveness rose when the delay between the pulses surpassed the refractory periods of these cells. Our inference was that greater currents produced progressively overlapping stimulation fields. Moreover, evidence of overlap was seen at lower currents in rats that had been prepared with smaller separations between the electrodes. Our estimate of the threshold current density for the most sensitive of the reward units is 1300 microA/mm2 when 0.1 ms pulses are used.