and phenylalanine together, though not singly; without arginine growth was very slight. The remaining amino acids found to be stimulatory in a fluid medium were omitted without effect from the semisolid agar medium. The need for heavy inocula to initiate growth in both fluid and semisolid media and our inability to culture the organism serially in the liquid medium suggest additional growth essentials. This is borne out by the fact that washed cells grew better in a fluid peptone medium than in the synthetic medium and by the ability of the organism to grow on serial transfer in the former. The replacement of agar by other substances providing increased surface, such as glass wool, crushed glass, and glass beads, was ineffective in stimulating growth, as was aeration.

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Endo (1904) suggested the use of a medium of the following composition for the differentiation of B. typ wu from B. colilike organisms:

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