No abstract

The current study tested the hypothesis that exposure to hypoxia enhances endothelin-1 (ET-1) gene expression and elevates circulating ET-1 levels in the rat. Rats were exposed to normobaric hypoxia (10% O2) or room air for 24 or 48 h. ET-1 in arterial blood was measured by radioimmunoassay. ET-1 gene transcript levels were measured by the slot blot technique on total RNA isolated from lung, right and left atria, right and left ventricles, kidney, spleen, liver, brain, main trunk of pulmonary artery, and thoracic aorta. Blots were probed with a 0.5 kb rat prepro ET-1 cDNA that does not cross-hybridize with mRNA for ET-2 or ET-3. Plasma ET-1 levels were increased significantly at 24 (10.03 +/- 2.33 pg/ml) and 48 h (14.02 +/- 3.44 pg/ml) of hypoxia compared with air controls (4.14 +/- 0.66 pg/ml). ET-1 mRNA levels were increased significantly (2-fold) in lung and right atrium after 48 h of hypoxia; no change was seen in organs perfused by the systemic vascular bed. These findings suggest that the hypoxia-induced increase in circulating ET-1 levels is mainly of pulmonary origin. A paracrine effect of ET-1 produced by lung endothelial cells could account for hypoxic pulmonary hypertension.

Results of this study provide a "Model of HIV Disclosure" that can be utilized by nurse practitioners and other HCPs in clinical practice when providing treatment, counseling, and prevention education for HIV+ clients and for prevention education for HIV negative clients--especially for family, friends, sexual partners, and employers of HIV+ clients.

No abstract

Seismic images of active fault zones can be used to examine the structure of faults throughout the crust and upper mantle and give clues as to whether the associated deformation occurs within a narrow shear zone or is broadly distributed through the lower crust. Limitations on seismic resolution within the crust and difficulties imaging shallow structures such as the crust‐mantle boundary (Moho) place constraints on the interpretation of seismic images. In this study we retrieve body wave reflections from autocorrelations of ambient seismic noise. The instantaneous phase coherence autocorrelations allow unprecedented ambient noise images of the North Anatolian Fault Zone (NAFZ). Our reflection profiles show a Moho reflected P wave and additional structure within the crust and upper mantle. We image a distinct vertical offset of the Moho associated with the northern branch of the NAFZ indicating that deformation related to the fault remains narrow in the upper mantle.