Context West African crocodylian populations are declining and in need of conservation action. Surveys and other monitoring methods are critical components of crocodile conservation programs; however, surveys are often hindered by logistical, financial and detectability constraints. Increasingly used in wildlife monitoring programs, drones can enhance monitoring and conservation efficacy. Aims This study aimed to determine a standard drone crocodylian survey protocol and evaluate the drones as a tool to survey the diverse crocodylian assemblage of West Africa. Methods We surveyed crocodile populations in Benin, Côte d’Ivoire, and Niger in 2017 and 2018, by using the DJI Phantom 4 Pro drone and via traditional diurnal and nocturnal spotlight surveys. We used a series of test flights to first evaluate the impact of drones on crocodylian behaviour and determine standard flight parameters that optimise detectability. We then, consecutively, implemented the three survey methods at 23 sites to compare the efficacy of drones against traditional crocodylian survey methods. Key results Crocodylus suchus can be closely approached (>10 m altitude) and consumer-grade drones do not elicit flight responses in West African large mammals and birds at altitudes of >40–60 m. Altitude and other flight parameters did not affect detectability, because high-resolution photos allowed accurate counting. Observer experience, field conditions (e.g. wind, sun reflection), and site characteristics (e.g. vegetation, homogeneity) all significantly affected detectability. Drone-based crocodylian surveys should be implemented from 40 m altitude in the first third of the day. Comparing survey methods, drones performed better than did traditional diurnal surveys but worse than standard nocturnal spotlight counts. The latter not only detected more individuals, but also a greater size-class diversity. However, drone surveys provide advantages over traditional methods, including precise size estimation, less disturbance, and the ability to cover greater and more remote areas. Drone survey photos allow for repeatable and quantifiable habitat assessments, detection of encroachment and other illegal activities, and leave a permanent record. Conclusions Overall, drones offer a valuable and cost-effective alternative for surveying crocodylian populations with compelling secondary benefits, although they may not be suitable in all cases and for all species. Implications We propose a standardised and optimised protocol for drone-based crocodylian surveys that could be used for sustainable conservation programs of crocodylians in West Africa and globally.

The management schemes of four rain forest patches in southern Benin and south-western Nigeria, which led to the successful protection of numerous threatened plants and animals over the last 20 plus years, are analysed. Since climatic conditions are similar, tree composition depends largely on different availability of water and documented biodiversity mostly on the availability of taxonomic expertise. Management differs according to accessibility and human population pressure, from total closing off of the forest by an international institute near the mega-polis Ibadan to unmarked borders near Lanzron, a remote village in the lower Ouémé Valley, where foreigners are mostly excluded from visiting the site. In Benin, trees and wildlife (antelopes and monkeys) seem best protected where the local vodoun beliefs are adhered to. This is, however, not sufficient and development aid to support and benefit the local population is needed as exemplified in Zinvié. At the Ibadan and Drabo sites, long-term protection is assured by legally-binding landtitles. Since for all of Lanzron and part of Zinvié these are lacking securing them is a priority. In Ibadan, Nigeria, a major rehabilitation effort is concentrated on bringing relatively old grass land and former village sites under forest cover by planting local trees. Rehabilitation in Drabo, in southern Benin, relies on enriching the naturally occurring fallow succession with rare species from nearby threatened sacred forests. We demonstrate that reversing biodiversity loss is possible but requires a long-term commitment. Recommendations for protecting, stabilizing and enhancing similar small hotspots of biodiversity are made. RESEARCH ARTICLE Launched to accelerate biodiversity conservation A peer-reviewed open-access journalPeter Neuenschwander et al. / Nature Conservation 13: 21-46 (2015) 22

Ticks and tick-borne pathogens constitute a growing veterinary and public health concern around the world. Ticks are considered to be natural reservoirs for tick borne related pathogens and are equally responsible for the spread of infections in animals as well as in humans. In this study, the presence of Rickettsia and Bartonella species and Anaplasma phagocytophilum was investigated in hard ticks collected from reptiles, birds and wild mammalian animals. The samples collection of this study was carried out between December 2020 and September 2021. Adult ticks (male and female) were collected from wild animals in six districts of southern Benin. Molecular analysis was used to verify the presence of pathogens in all the samples (ticks) collected from wild animals. A total of 504 ticks were collected and grouped into 115 different tick pools. The PCR analysis detected 19 out of 115 tick pools positive for Rickettsia spp. and 9/115 tick pools positive for Bartonella spp., while Anaplasma phagocytophilum DNA was not detected in any tick. Several of the tick species collected from our studied reptiles/wild mammalian animals could be potential sources of zoonotic pathogens when subjected to further investigation. Therefore, stringent attention should be paid to tick infestation of reptiles/wild mammalian animals in order to put in place proper control and prevention measures for tick-borne diseases in the wild, which could serve as reservoirs in the infestation of domestic animals/humans in the event of any possible contact.

Ticks and tick-borne pathogens constitute a growing veterinary and public health concern around the world. Ticks are considered to be natural reservoirs for tick borne related pathogens and are equally responsible for the spread of infections in animals as well as in humans. In this study, the presence of Rickettsia and Bartonella species and Anaplasma phagocytophilum was investigated in hard ticks collected from reptiles, birds and wild mammalian animals. The samples collection of this study was carried out between December 2020 and September 2021. Adult ticks (male and female) were collected from wild animals in six districts of southern Benin. Molecular analysis was used to verify the presence of pathogens in all the samples (ticks) collected from wild animals. A total of 504 ticks were collected and grouped into 115 different tick pools. The PCR analysis detected 19 out of 115 tick pools positive for Rickettsia spp. and 9/115 tick pools positive for Bartonella spp., while Anaplasma phagocytophilum DNA was not detected in any tick. Several of the tick species collected from our studied reptiles/wild mammalian animals could be potential sources of zoonotic pathogens when subjected to further investigation. Therefore, stringent attention should be paid to tick infestation of reptiles/wild mammalian animals in order to put in place proper control and prevention measures for tick-borne diseases in the wild, which could serve as reservoirs in the infestation of domestic animals/humans in the event of any possible contact.