This study was conducted to determine if the acute: chronic workload ratio (ACWR) is related to the incidence of non-contact injuries. The purpose is to compare the external load of injured and non-injured soccer players with the same characteristics, such as position and age. The present analysis considers both the four and the two weeks preceding an injury. Physical characteristics were recorded and analyzed through global positioning systems (GPS) evaluation over one season of 24 competitive microcycles, 144 training sessions, and 32 matches in a total of 35 professional soccer players from the Greek Super League 1 and Super League 2. The loads calculated were total distance (TD), 15–20 km/h, 20–25 km/h, 25–30 km/h, accelerations (ACC) > 2.5 m/s2, and decelerations (DEC) > 2.5 m/s2). Nine injured athletes exceeded the critical threshold of an ACWR > 1.3 several times compared with non-injured athletes that did not reach this level. The present study showed that ACWR is related to a subsequent occurrence of injury but that the threshold of an ACWR can vary. This seems to be mainly influenced by assessing the load of the last two weeks compared with that of the four weeks before the injury.

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