Objective:The aim of this study was to evaluate the viability of human periodontal ligament (PDL) cells on MTA-Fillapex, GuttaFlow 2, TotalFill Sealer, and BioRoot™ RCS in comparison to conventional epoxy resin-based (AH Plus) and zinc oxide-eugenol-based (Roth's 801) sealers.Materials and Methods:Sealers were divided into two groups, and five coverslips for each material per group were prepared. In the first group, PDLs were added immediately after the preparation of sealers (Fresh Group), and in the second, PDLs were added after 24 h. PDLs were cultured for 72 h and afterward, counted using standard hematocytometry. A Mann–Whitney U-test and Kruskal–Wallis test were used for the statistical analysis. The level of significance was set at 5%. Furthermore, cell morphology was assessed by confocal microscopy.Results:The number of viable cells for the 24 h-set groups was higher than the freshly mixed in all sealers except Roth's 801. In both groups, GuttaFlow 2 presented the highest number of viable cells. In a descending order of cells' survival, TotalFill, BioRoot, and MTA-Fillapex are following and the conventional sealers, AH Plus and Roth's 801, seem not to exhibit the biological properties of the others. Cells grown on GuttaFlow 2, TotalFill, and BioRoot were observed to be well-formed. In contrast, MTA-Fillapex exhibited untypical morphology. No cells were detected on the surfaces of AH Plus, as well as Roth's 801.Conclusions:All novel sealers presented increased cell viability in comparison to conventional sealers. GuttaFlow 2 exhibited the highest cell viability.

The purpose of this study was to explore the level of agreement between the radiographic and the electronic apex locator (EAL) working length assessment methods, amongst teeth with different diagnoses. Working length measurements along with pulpal and periapical diagnoses data were collected from the Patient Registry Database of our Institution. The null hypothesis of this study was that pulp status does not affect the level of agreement between the two methods. The degree of agreement was assessed using the Bland-Altman method, followed by a Kruskal-Wallis test (a = 5%) that would allow the null hypothesis to be accepted. The EAL measurements agree in general with those provided by a 1.5 mm file placed coronal to the radiographic apex amid teeth with different pulpal diagnoses. Within the limitations of this study, we suggest that this setting (i.e. the distance) may be applied to all cases of endodontically treated teeth, despite the differences in pulpal diagnosis.