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Adult male Sprague-Dawley rats were orally administered 14C-alternariol monomethyl ether (AME) in olive oil and housed in glass metabolism cages. Urine, feces, and expired CO2 were monitored for the presence of radioactivity. The majority of activity was excreted in the feces apparently unabsorbed. Urine excretion accounted for less than 10% of the administered dose. A substantial amount of "absorbed" activity was excreted in expired CO2. TLC autoradiography of urine extracts revealed extensive metabolism, but little metabolism was evident in feces extracts. Incubation of AME with rat liver post-mitochondrial supernatant also resulted in substantial metabolism.

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The 7-d urinary and fecal excretions of [14C]toxaphene and two isolated [14C]toxaphene fractions (polar fraction 7 and nonpolar fraction 2) were determined in orally dosed rats. The urinary, fecal, and total excretions of toxaphene were, respectively, 22.5, 35.7, and 58.2% of the administered dose. The total excretions of fractions 2 and 7 were, respectively, 69.4 and 65.0%, and the overall order of excretion was fraction 2 greater than toxaphene greater than fraction 7. All three groups had low toxaphene levels (below 0.2 ppm) in all tissues analyzed except for fat, where significant levels were detected. Hexane and chloroform extracts of the urine revealed that the activity was more polar than the parent material for all three groups. Apparently, toxaphene must be metabolized before it can be excreted in the urine. When fat extracts were analyzed by thin-layer chromatography and autoradiography, differences were found between the parent material and the extracted activity. There was an increase in polar activity in the residue obtained from toxaphene-treated rats. The fat from the fraction 2 group contained fraction 2 and two additional more polar spots, which represented about 11% of the total activity. The fat from the fraction 7 group also contained two additional spots, but they were less polar than fraction 7. Apparently, the metabolism of fraction 7 results in some products that are less polar and, perhaps, more persistent.